Kiel Network of Analytical Spectroscopy and Mass Spectrometry

Platform Overview

Within the network and the involved groups, different analytical spectroscopy and mass spectrometry systems are present. In the following, the different systems are shortly presented and described to get an overview about the analytical possibilities and cooperation.

Instruments of the Otto-Diels-Institute for Organic Chemistry - Spectroscopy

Major capabilties  are:

1D- and 2D NMR spectroscopy: (individual bullets)

  •  full structure elucidation and verification with correlation experiments
  • determination of diffusion constants/DOSY experiments and estimation of MW
  • chemical exchange spectroscopy
  • determination of relaxation rates and relaxivities
  • Evans measurements
  • determination of reaction rates
  • quantification of molecular interactions (i.e. protein-inhibitor interactions, STD)
  • mixture analyses


MS-spectroscopy:

  •  (high resolution) mass determination
  •  MS^n spectroscopy
  •  MALDI, EI, FD, FI, LIFDI and ESI ionisation techniques

Instruments at the Division of Food Technology

Fourier transform ion cyclotron resonance – mass spectrometry systems (FT-ICR)

  • Direct injection (Shotgun) or linkable to an UHPLC system
  • ESI, APCI & MALDI- sources
  • ETD/ECD-Option
  • Mass precision: <1 ppm
  • Resolution up to 600.000    

 

Quadrupole Time-of-Flight System (QToF)        

  • Direct injection (Shotgun) or linkable to an UHPLC system 
  • ESI & APCI - sources
  • Mass precision: 30 ppm
  • Resolution up to 10.000

 

Premier XE - Triple Quadrupole (TQ)

  • Direct injection (Shotgun) or linkable to an UHPLC system
  • ESI - source                 

 

HPLC-systems

  • two semi-UHPLC-systems (Agilent, Infinity 1260, equipped with an DAD)
  • two HPLC-systems (Agilent, HP1100, equipped with DAD or fluorescent-detector)
  • preparative HPLC (Agilent, equipped with an DAD)
  • one UHPLC (Dionex, Ultimate 3000)

 

GC-systems

  • one equipped with a liquid injection (FID)
  • one equpped with a head-space autosampler
  • one GC-MS with SPME, static and dynamic headspace

 

Electron paramagnetic resonance spectroscopy system

Instruments of the Systematic Proteomics and Bioanalytics

Instrumentation:

  • ESI Orbitrap Velos with electron transfer dissociation (ETD) (Thermo)
  • ESI QExactive Plus (Thermo)
  •  ESI/MALDI Synapt G2S, with ion mobility (Waters)
  • 5800 MALDI TOF/TOF MS (ABSciex)
  • ESI 3000- Triple Quadrupole MS (ABSciex)
  • nano-LC (U3000) (Dionex)
  • UPLC- 3000 RSLC (Dionex)
  • Analytical LC System
  • Probot for LC-MALDI MS
  • in-house bioinformatics (Raid, NAS)

 

Institute of Plant Nutrition and Soil Science

At the Institute of Plant Nutrition and Agrology different kinds of mass spectrometry systems are available und can be used e.g. for cooperation. Two examples of these systems are followed. A detailed description will be available in the next time.

1) Liquid Chromatography - Inductive Linked Plasma - mass spectrometer (LC-ICP-MS)

2) Stable isotope - mass spectrometer (IRMS)


For more informations, please contact Prof. Dr. Mühling (e-mail) or Dr. Britta Pitann ([e-mail)

Department of Safety and Quality of Milk and Fish Products, MRI

  • Linear iontrap with electrospray ionisation (ESI) and electron transfer dissociation (ETD) (LTQXL, ThermoFisherScientific) coupled with nHPLC (Ultimate 3000, RSLC nanoSystem) or ion chromatography system (ICS 3000)
  • peptides and proteins in milk and fish products
  • carbohydrates and milk oligosaccharides in milk of different mammals
  • High Resolution Sector Field Mass Spectrometry (HRMS)
    • polychlorinated dibenzodioxins/-furans and polychlorinated biphenyls
  • Stable Isotope Ratio Mass Spectrometry (IRMS)
    • 13C and d15N in organic solids